Mental Health, Quality of Life and Coping Strategies in Vulnerable Children During the COVID-19 Pandemic.

BACKGROUND: The coronavirus pandemic has led to a situation without precedent in modern history. The aim of this study is to analyse the consequences after one year of the pandemic on a group of children and adolescents assessed at the beginning of the pandemic in 2020 and to determine the most effective ways of psychologically coping with this pandemic. METHOD: Two different, but equivalent, groups with a total of 604 (study I, 2020) and 743 (study II, 2021) children and adolescents in residential care, foster families, kinship families or family strengthening programs in Spain were evaluated using the SDQ (mental health measure), KIDSCREEN-10 index (quality of life measure) and Kidcope (coping behaviour measure). An independent sample t-test and a decision tree analysis were used. RESULTS: The mental health of children and adolescents decreased by 9.7%, and Self-Perceived quality of life did not change after one year of the COVID-19 pandemic. Nonactive coping strategies predicted worse mental health and worse quality of life. Problem solving served as a protective factor. CONCLUSION: One year after, the COVID-19 pandemic has an effect on the psychological wellbeing of children and adolescents, and the consequences can be reduced if proper coping strategies are used.

Climatic stability, not average habitat temperature, determines thermal tolerance of subterranean beetles.

The climatic variability hypothesis predicts the evolution of species with wide thermal tolerance ranges in environments with variable temperatures, and the evolution of thermal specialists in thermally stable environments. In caves, the extent of spatial and temporal thermal variability experienced by taxa decreases with their degree of specialization to deep subterranean habitats. We use phylogenetic generalized least squares to model the relationship among thermal tolerance (upper lethal limits), subterranean specialization (estimated using ecomorphological traits), and habitat temperature in 16 beetle species of the tribe Leptodirini (Leiodidae). We found a significant, negative relationship between thermal tolerance and the degree of subterranean specialization. Conversely, habitat temperature had only a marginal effect on lethal limits. In agreement with the climatic variability hypothesis and under a climate change context, we show that the specialization process to live in deep subterranean habitats involves a reduction of upper lethal limits, but not an adjustment to habitat temperature. Thermal variability seems to exert a higher evolutionary pressure than mean habitat temperature to configure the thermal niche of subterranean species. Our results provide novel insights on thermal physiology of species with poor dispersal capabilities and on the evolutionary process of adaptation to subterranean environments. We further emphasize that the pathways determining vulnerability of subterranean species to climate change greatly depend on the degree of specialization to deep subterranean environments.

Mental Health, Quality of Life and Coping Strategies in Vulnerable Children During the COVID-19 Pandemic / Salud Mental, Calidad de Vida y Estrategias de Afrontamiento de Niños Vulnerables Durante la Pandemia de la COVID-19

Background: The coronavirus pandemic has led to a situation withoutprecedent in modern history. The aim of this study is to analyse theconsequences after one year of the pandemic on a group of children andadolescents assessed at the beginning of the pandemic in 2020 and todetermine the most effective ways of psychologically coping with thispandemic. Method: Two different, but equivalent, groups with a total of604 (study I, 2020) and 743 (study II, 2021) children and adolescents inresidential care, foster families, kinship families or family strengtheningprograms in Spain were evaluated using the SDQ (mental health measure),KIDSCREEN-10 index (quality of life measure) and Kidcope (copingbehaviour measure). An independent sample t-test and a decision treeanalysis were used.Results: The mental health of children and adolescentsdecreased by 9.7%, and Self-Perceived quality of life did not change afterone year of the COVID-19 pandemic. Nonactive coping strategies predictedworse mental health and worse quality of life. Problem solving served asa protective factor. Conclusion: One year after, the COVID-19 pandemichas an effect on the psychological wellbeing of children and adolescents,and the consequences can be reduced if proper coping strategies are used.

Antecedentes: la pandemia del coronavirus no tiene precedentes en la historia moderna. El objetivo del estudio es analizar sus consecuencias tras un año de pandemiaen niños y adolescentes evaluados al inicio de la misma en 2020 y determinarlas estrategias de afrontamiento más eficaces para lidiar con la pandemia.Método: 2 grupos distintos pero equivalentes formados por 604 (estudioI, 2020) y 743 (estudio II, 2021) niños y adolescentes en acogimientoresidencial, acogimiento familiar (extensa y ajena) y en programas de fortalecimiento familiar en España fueron evaluados usando el SDQ (saludmental), KIDSCREEN-10 (calidad de vida) y Kidcope (afrontamiento). Se utilizó comparaciones de medias para muestras independientes y unanálisis de árbol de decisión. Resultados: la salud mental de niños yadolescentes ha disminuido un 9,7% mientras que la calidad de vida no hacambiado tras un año de pandemia. Las estrategias de afrontamiento pasivaspredijeron una peor salud mental y una peor calidad de vida. Estrategias desolución de problemas actuaron como un factor protector. Conclusiones: la pandemia de la COVID-19 tiene efectos tras el paso de un año en elbienestar psicológico de niños y adolescentes y sus consecuencias puedenreducirse con estrategias de afrontamiento apropiadas.

A redefinition of the genus Troglocharinus Reitter, 1908, with description of new species (Coleoptera, Leiodidae, Cholevinae, Leptodirini).

We redefine the genus Troglocharinus Reitter, 1908 based on a phylogenetic analysis with a combination of mitochondrial and molecular data. We recovered the current Speonomites mengeli (Jeannel, 1910) and S. mercedesi (Zariquiey, 1922) as valid, separate species within the Troglocharinus clade, not directly related to Speonomites Jeannel, 1910, a finding corroborated by a detailed study of the male and female genitalia. In consequence, we reinstate Speonomus mercedesi Zariquiey, 1922 stat. nov. as a valid species, transfer both of them to the genus Troglocharinus, T. mengeli (Jeannel, 1910) comb. nov. and T. mercedesi (Zariquiey, 1922) comb. nov., and redescribe the genus. The study of new material from the distribution area of the former S. mengeli revealed the presence of two undescribed species, T. sendrai sp. nov. and T. fadriquei sp. nov., which we describe herein. We designate the lectotype of Speonomus vinyasi Escolà, 1971 to fix its identity, as among its syntypes there are two different species. In agreement with the results of the phylogenetic analyses we establish the synonymy between the genus Speonomites and Pallaresiella Fresneda, 1998 syn. nv.

Psychological Wellbeing of Vulnerable Children During the COVID-19 Pandemic / Bienestar Psicológico de Niños Vulnerables Durante la Pandemia del COVID-19

BACKGROUND: A pandemic disaster has specific effects on mental health, however, little is known about those specific effects in children and adolescents. Thus, the aim of this study is to describe the psychological impact of the COVID-19 pandemic on a sample of children and adolescents and to compare the results with previous national data and other studies to determine variations. METHOD: A total of 459 children and adolescents in residential care, foster families, kinship families, or family strengthening programs under SOS Children's Villages Spain were evaluated using the SDQ to measure internalizing and externalizing problems and using KIDSCREEN-10 index to measure heath related quality of life. An independent sample t-test, one-way ANOVA and the chi-square test were used. RESULTS: The children and adolescents in this study had worse psychological wellbeing than those in the 2017 Spanish reference, that is, before the COVID-19 outbreak. Quality of life remained the same. No differences between care modalities were found. CONCLUSION: It is necessary to monitor the mental health status of children and adolescents to prevent possible problems. Additionally, it is necessary to use well-known assessment instruments because it is essential to have a reference to other situations and populations

ANTECEDENTES: una pandemia tiene efectos concretos sobre la salud mental; sin embargo, dichos efectos son poco conocidos en niños y adolescentes. El objetivo de este estudio es describir el impacto psicológico de la COVID-19 en una muestra de niños y adolescentes y comparar los resultados con datos de referencia nacionales y otros estudios. MÉTODO: un total de 459 niños y adolescentes en acogimiento residencial, familias de acogida extensa y ajena y en programas de fortalecimiento familiar, atendidos por Aldeas Infantiles SOS España, fueron evaluados mediante el SDQ para determinar la presencia de problemas internalizantes y externalizantes y mediante el KIDSCREEN-10 para determinar su nivel de calidad de vida. Se utilizó comparaciones de medias para muestras independientes, ANOVA y Chi-cuadrado. RESULTADOS: los niños y adolescentes estudiados obtuvieron peores resultados en bienestar psicológico que los publicados como referencia nacional en España en 2017, antes del brote de la COVID-19. La calidad de vida no se ha visto afectada. No se han encontrado diferencias entre las modalidades de cuidado. CONCLUSIONES: es necesario evaluar la salud mental de los niños y adolescentes para prevenir posibles problemas. Adicionalmente, es necesario usar instrumentos bien conocidos que permitan comparar los resultados con otras situaciones y poblaciones

Psychological Wellbeing of Vulnerable Children During the COVID-19 Pandemic.

BACKGROUND: A pandemic disaster has specific effects on mental health, however, little is known about those specific effects in children and adolescents. Thus, the aim of this study is to describe the psychological impact of the COVID-19 pandemic on a sample of children and adolescents and to compare the results with previous national data and other studies to determine variations. METHOD: A total of 459 children and adolescents in residential care, foster families, kinship families, or family strengthening programs under SOS Children's Villages Spain were evaluated using the SDQ to measure internalizing and externalizing problems and using KIDSCREEN-10 index to measure heath related quality of life. An independent sample t-test, one-way ANOVA and the chi-square test were used. RESULTS: The children and adolescents in this study had worse psychological wellbeing than those in the 2017 Spanish reference, that is, before the COVID-19 outbreak. Quality of life remained the same. No differences between care modalities were found. CONCLUSION: It is necessary to monitor the mental health status of children and adolescents to prevent possible problems. Additionally, it is necessary to use well-known assessment instruments because it is essential to have a reference to other situations and populations.

A molecular phylogeny of Speonemadus Jeannel, 1922 with description of two new species from Morocco (Coleoptera: Leiodidae: Cholevinae: Anemadini).

The genus Speonemadus Jeannel, 1922 currently includes 12 species distributed in the Western Mediterranean (Iberian Peninsula, North Africa, Sicily and continental Italy). Two new species are described from Morocco, S. brusteli sp. n. from the Rif and S. comasi sp. n. from the High Atlas, the first in the S. vandalitiae-group of species and the second with an isolated position within the genus. Two species are also resurrected from synonymy, S. pulchellus (Reitter, 1885) stat. rest. (former synonym of S. orchesioides (Fairmaire, 1879)) and S. gracilis (Kraatz, 1870) stat. rest. (former synonym of S. vandalitiae (Heyden, 1870)), raising the total number of species of the genus to 16. Lectotypes are designated for Anemadus pulchellus Reitter, 1885, and Anemadus tenuipes Peyerimhoff, 1917. A molecular phylogeny of the genus Speonemadus is presented, based on a combination of mitochondrial and nuclear genes and including seven of the currently recognised species, one of the newly described (S. brusteli sp. n.) and the two reinstated taxa. The genus is estimated to have diversified from the late Miocene (Tortonian), with successive cladogenetic events related to the isolation of landmasses between Iberia and North Africa, including the opening of the Strait of Gibraltar at the end of the Messinian. The distribution of some of the species of Speonemadus remains still uncertain, due to their frequent misidentification and the problems with their taxonomic status.

On the genus Aphaobius Abeille de Perrin, 1878, with description of a new species from the mesovoid shallow substratum (MSS) of Austria (Coleoptera: Leiodidae: Cholevinae: Leptodirini).

Aphaobius haraldi sp. n. from the mesovoid shallow substratum (MSS) of the Austrian Alps is described, illustrated, and compared with the closest species of the genus. The new species belongs to the A. kraussi species group, formerly including five species. It can be readily separated from other species of the genus by the large parameres, with an enlarged apical part, a unique feature among species of Aphaobius. The phylogenetic position of the new species is clarified using mitochondrial and nuclear data of four related species of Aphaobius, plus some representatives of related genera from the same geographic area. The diversification of the group was estimated to be recent, dating from the Pleistocene. New records and molecular data are provided regarding the enigmatic monospecific genus of the Austrian Alps, Lotharia Mandl, 1944, which was found to be sister to the studied species of Aphaobius.

Thermal niche estimators and the capability of poor dispersal species to cope with climate change.

For management strategies in the context of global warming, accurate predictions of species response are mandatory. However, to date most predictions are based on niche (bioclimatic) models that usually overlook biotic interactions, behavioral adjustments or adaptive evolution, and assume that species can disperse freely without constraints. The deep subterranean environment minimises these uncertainties, as it is simple, homogeneous and with constant environmental conditions. It is thus an ideal model system to study the effect of global change in species with poor dispersal capabilities. We assess the potential fate of a lineage of troglobitic beetles under global change predictions using different approaches to estimate their thermal niche: bioclimatic models, rates of thermal niche change estimated from a molecular phylogeny, and data from physiological studies. Using bioclimatic models, at most 60% of the species were predicted to have suitable conditions in 2080. Considering the rates of thermal niche change did not improve this prediction. However, physiological data suggest that subterranean species have a broad thermal tolerance, allowing them to stand temperatures never experienced through their evolutionary history. These results stress the need of experimental approaches to assess the capability of poor dispersal species to cope with temperatures outside those they currently experience.

Lack of evolutionary adjustment to ambient temperature in highly specialized cave beetles.

BACKGROUND: A key question in evolutionary biology is the relationship between species traits and their habitats. Caves offer an ideal model to test the adjustment of species to their surrounding temperature, as they provide homogeneous and simple environments. We compared two species living under different thermal conditions within a lineage of Pyrenean beetles highly modified for the subterranean life since the Miocene. One, Troglocharinus fonti, is found in caves at 4-11°C in the ancestral Pyrenean range. The second, T. ferreri, inhabits the coastal area of Catalonia since the early Pliocene, and lives at 14-16°C. RESULTS: We found no differences in their short term upper thermal limit (ca. 50°C), similar to that of most organisms, or their lower thermal limit (ca. -2.5°C), higher than for most temperate insects and suggesting the absence of cryoprotectants. In longer term tests (7 days) survival between 6-20°C was almost 100% for both species plus two outgroups of the same lineage, but all four died between 23-25°C, without significant differences between them. CONCLUSIONS: Our results suggest that species in this lineage have lost some of the thermoregulatory mechanisms common in temperate insects, as their inferred default tolerance range is larger than the thermal variation experienced through their whole evolutionary history.

Developmental constraints in cave beetles.

In insects, whilst variations in life cycles are common, the basic patterns typical for particular groups remain generally conserved. One of the more extreme modifications is found in some subterranean beetles of the tribe Leptodirini, in which the number of larval instars is reduced from the ancestral three to two and ultimately one, which is not active and does not feed. We analysed all available data on the duration and size of the different developmental stages and compared them in a phylogenetic context. The total duration of development was found to be strongly conserved, irrespective of geographical location, habitat type, number of instars and feeding behaviour of the larvae, with a single alteration of the developmental pattern in a clade of cave species in southeast France. We also found a strong correlation of the size of the first instar larva with adult size, again regardless of geographical location, ecology and type of life cycle. Both results suggest the presence of deeply conserved constraints in the timing and energy requirements of larval development. Past focus on more apparent changes, such as the number of larval instars, may mask more deeply conserved ontogenetic patterns in developmental timing.

Life-history specialization was not an evolutionary dead-end in Pyrenean cave beetles.

Research on subterranean organisms has focused on the colonization process and some of the associated phenotypic changes, but little is known on the long-term evolutionary dynamics of subterranean lineages and the origin of some highly specialized complex characters. One of the most extreme modifications is the reduction of the number of larval instars in some Leptodirini beetles from the ancestral 3 to 2 and ultimately a single instar. This reduction is usually assumed to have occurred independently multiple times within the same lineage and geographical area, but its evolution has never been studied in a phylogenetic framework. Using a comprehensive molecular phylogeny, we found a low number of independent origins of the reduction in the number of instars, with a single transition, dated to the Oligocene-Miocene, from 3 to 2 and then 1 instar in the Pyrenees, the best-studied area. In the Pyrenees, the 1-instar lineage had a diversification rate (0.22 diversification events per lineage per million years) significantly higher than that of 3- or 2-instar lineages (0.10), and similar to that seen in other Coleopteran radiations. Far from being evolutionary dead-ends, ancient lineages fully adapted to subterranean life seem able to persist and diversify over long evolutionary periods.

Discovery of a new blind ground beetle in western French Pyrenees, and its relevance to the phylogeny of Pyrenean hypogean Trechini.

Geotrechus sarpedon sp. n., a new species of blind Trechini is described from Arbailles Massif, western Pyrenees, trance. The generic attribution of the new species was challenging as it shares morphological features of both genera Geotrechus Jeannel, 1919 and Aphaenops subgenus Hydraphaenops Jeannel, 1926, confirming their polyphyly, which has been highlighted by previous molecular studies on the group. Molecular data suggests unambiguously strong affinities between the new species and the clade Aphaenops vasconicus Jeannel, 1913-A. galani Espanol, 1968 occurring in the same geographic area, and not related with Aphaenops ehlersi (Abeille de Perrin, 1872), type species of the subgenus Hydraphaenops. The species is included in the paraphyletic genus Geotrechus until its affinities with the other species of Geotrechus and A. (Hydraphaenops) are clarified.

Molecular phylogeny of the Trechus brucki group, with description of two new species from the Pyreneo-Cantabrian area (France, Spain) (Coleoptera, Carabidae, Trechinae).

A molecular phylogeny of the species from the Trechus brucki clade (previously Trechus uhagoni group)based on fragments of four mitochondrial genes and one nuclear gene is given. We describe Trechus (Trechus) bouillonisp. n. from the western pre-Pyrenees: Sierras de Urbasa-Andía, Navarra, Spain. The species was collected in mesovoid shallow substratum (mss), a subterranean environment. Molecular as well as morphological evidences demonstrate that the new species belongs to the Trechus brucki clade. A narrow endemic species of high altitude in western French Pyrenees merged with Trechus brucki Fairmaire, 1862a, Trechus bruckoidessp. n., is described. A lectotype is designated for Trechus brucki and Trechus planiusculus Fairmaire, 1862b (junior synonym of Trechus brucki). The species group is redefined based on molecular and morphological characters, and renamed as the brucki group, as Trechus brucki was the first described species of the clade. A unique synapomorphy of the male genitalia, a characteristic secondary sclerotization of the sperm duct, which is shared by all the species of the brucki group sensu novo, is described and illustrated. The Trechus brucki group sensu novo is composed of Trechus beusti (Schaufuss, 1863), Trechus bouillonisp. n., Trechus brucki, Trechus bruckoidessp. n., Trechus grenieri Pandellé, 1867, T. uhagoni uhagoni Crotch, 1869, T. uhagoni ruteri Colas, 1935 and Trechus pieltaini Jeannel, 1920. We discuss the taxonomy of the group and provide illustrations of structures showing the differences between the species, along with distribution data and biogeographical comments.

Ancient origin of a Western Mediterranean radiation of subterranean beetles.

BACKGROUND: Cave organisms have been used as models for evolution and biogeography, as their reduced above-ground dispersal produces phylogenetic patterns of area distribution that largely match the geological history of mountain ranges and cave habitats. Most current hypotheses assume that subterranean lineages arose recently from surface dwelling, dispersive close relatives, but for terrestrial organisms there is scant phylogenetic evidence to support this view. We study here with molecular methods the evolutionary history of a highly diverse assemblage of subterranean beetles in the tribe Leptodirini (Coleoptera, Leiodidae, Cholevinae) in the mountain systems of the Western Mediterranean. RESULTS: Ca. 3.5 KB of sequence information from five mitochondrial and two nuclear gene fragments was obtained for 57 species of Leptodirini and eight outgroups. Phylogenetic analysis was robust to changes in alignment and reconstruction method and revealed strongly supported clades, each of them restricted to a major mountain system in the Iberian peninsula. A molecular clock calibration of the tree using the separation of the Sardinian microplate (at 33 MY) established a rate of 2.0% divergence per MY for five mitochondrial genes (4% for cox1 alone) and dated the nodes separating the main subterranean lineages before the Early Oligocene. The colonisation of the Pyrenean chain, by a lineage not closely related to those found elsewhere in the Iberian peninsula, began soon after the subterranean habitat became available in the Early Oligocene, and progressed from the periphery to the centre. CONCLUSIONS: Our results suggest that by the Early-Mid Oligocene the main lineages of Western Mediterranean Leptodirini had developed all modifications to the subterranean life and were already present in the main geographical areas in which they are found today. The origin of the currently recognised genera can be dated to the Late Oligocene-Miocene, and their diversification can thus be traced to Miocene ancestors fully adapted to subterranean life, with no evidence of extinct epigean, less modified lineages. The close correspondence of organismal evolution and geological record confirms them as an important study system for historical biogeography and molecular evolution.

Sex and body mass index specific regulation of blood pressure by CYP19A1 gene variants.

Sexual dimorphism in blood pressure (BP) regulation has been observed both in humans and experimental animals, and estrogens have been shown to contribute to this epidemiological observation. A key enzyme in determining estrogen levels is aromatase cytochrome P450. The aim of this study was to evaluate the role of the gene encoding aromatase, CYP19A1, as an independent risk factor for hypertension and its relationship with systolic and diastolic BP measures. We genotyped 2 polymorphisms within the CYP19A1 gene, IVS4 rs11575899 and 3'UTR rs10046, in 3448 individuals. In quantitative analysis, we observed significant associations between the 2 polymorphisms and BP values in women, being these associations dependent on BMI and independent of menopause status. The case-control analysis revealed that the most prominent associations were found for nonobese women in diastolic hypertension (DHT): the IVS4_22 and 3'UTR_11 are risk genotypes (OR=1.61, P=0.027 and OR=1.59, P=0.012, respectively), whereas IVS4_11 and 3'UTR_22 genotypes have a protective effect against DHT (OR=0.63, P=0.009, and OR=0.61, P=0.020, respectively). Haplotype analysis confirmed the above associations: among nonobese women the haplotype 21 is overrepresented in hypertensive women (OR=1.33, P=0.004, for DHT and OR=1.25, P=0.026, for systolic hypertension, SHT) and, conversely, the haplotype 12 protects against hypertension (OR=0.78, P=0.015 for DHT and OR=0.82, P=0.04 for SHT). Our study has shown that the CYP19A1 gene may be involved in the genetic regulation of BP in women. This effect is dependent on BMI and independent of menopause status, suggesting that this action is mainly driven by aromatase activity in fat tissue.